不同NFC/NDF比日粮对奶山羊瘤胃细菌及瘤胃和血浆中内毒素及组胺含量的影响

doi:10.11843/j.issn.0366-6964.2015.01.012

Abstract

Abstract:

This experiment was conducted to study the effect of increasing the ratio of non-fiber carbohydrate to neutral detergent fiber on bacteria flora，endotoxin and histamine content in rumen and plasma of dairy goats.Six rumen-cannulated lactating Guanzhong goats were used to investigate the effects of increasing dietary NFC/NDF ratio (i.e.non-fiber carbohydrate to neutral detergent fiber) on bacteria flora，and endotoxin and histamine contents in the rumen and the plasma of dairy goats.Induction of subacute ruminal acidosis (SARA) was performed by increasing dietary NFC/NDF ratio from 1.02(stage Ⅰ) to 1.24(stage Ⅱ)，1.63(stage Ⅲ) and 2.58(stage Ⅳ).Each stage lasted 10 days.A dynamic pH monitoring system and a rolling-tube method were used to assess changes in ruminal pH and the numbers of total bacteria，amylolytic bacteria and Fusobacterium necrophorum in rumen.Limulus test and fluorescence spectrophotometry were used to assess the contents of endotoxin and histamine.Our results showed that as the dietary NFC/NDF ratio increased from 1.02 to 2.58，the rumen pH decreased significantly (P<0.05) at accelerated rates，while the endotoxin and histamine contents in the rumen and the numbers of amylolytic bacteria and necrotic fusiform bacilli increased significantly (P<0.01 and P<0.05 respectively).We conclude that abnormal increases of endotoxin，histamine and F.necrophorum played an important role in the occurrence and development of SARA.

CLC Number:

GUO Peng,LIU Da-cheng，ZHAO Pei-ting，GAO Min，HU Hong-lian. Effects of Increasing the Ratio of Non-fiber Carbohydrate to Neutral Detergent Fiber on Bacteria Flora，Endotoxin and Histamine Content in Rumen and Plasma of Dairy Goats[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 96-103.

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Effects of Increasing the Ratio of Non-fiber Carbohydrate to Neutral Detergent Fiber on Bacteria Flora，Endotoxin and Histamine Content in Rumen and Plasma of Dairy Goats

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